Oral Biofilm Sampling for Microbiome Analysis in Healthy

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With paper points, the outer layer of subgingival plaque can be successfully cultured, but the deeper layer may be missed [10]. Background: The purpose of this study was to compare the results of microbiological RNA‐probe analysis after subgingival plaque sampling applying two different strategies. Methods: In 220 patients, c Subgingival plaque samples were taken from the mesio-buccal aspect of each tooth in each subject at each monitor-ing visit. Counts of 40 subgingival spe- cies wore determined in each plaque sample using the checkerboard DNA-DNA hybridization technique (Socran-sky et al. I994). After the removal of supragingival plaque, subgingival Two Subgingival Plaque‐Sampling Strategies Used With RNA Probes.

require training for proper collection; specific equipment and supplies required for sampling; More validation is needed. 29 Aug 2017 Patients with angiographic coronary artery disease with elevated low-density lipoprotein (LDL) cholesterol on chronic statin therapy were 28 Aug 2014 Ladders allow you to interpret the bands that you get in your sample lanes. Choose your ladder based on the expected band sizes. Always run leukocytes associated with the subgingival dental plaque samples.

15 Nov 2012 Dr. Buddy Mopper demonstrates a simple technique to close a subgingival margin of a composite restoration while maintaining the integrity of 21 Oct 2014 PAG set available on http://www.dental-pag-official.com the exclusive distributor.

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1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF. Comparative study of subgingival microbiological sampling techniques. Renvert S(1), Wikström M, Helmersson M, Dahlén G, Claffey N. Author information: (1)School of Dental Hygiene, Public Dental Service, Kristianstad, Sweden.

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However, the collection of saliva is simple and contains pathogens of all intraoral surfaces. The aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [MSP]; test) leads to results comparable with standard sampling method the subgingival microbiota, pathogens, and plaque [12]. In the present study, a gingival retraction cord (a specific dentistry tool) was used to sampling GCF and plaque: this is a novel sampling method that is potentially more reproducible and less technique-sensitive than the paper point sampling method. Curette sampling and a subgingival washing technique were compared within and between paired sites on two separate occasions in 24 patients using darkfield microscopy. Significantly lower mean proportions of spirochetes and motile rods and higher proportions of non-motile organisms were obtained with subgingival washing compared with curette sampling. Background: The purpose of this study was to compare the results of microbiological RNA‐probe analysis after subgingival plaque sampling applying two different strategies. Methods: In 220 patients, clinical examinations were obtained prior to commencement of therapy for aggressive or generalized severe chronic periodontitis (n = 113), after combined mechanical and antibiotic anti‐infective subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes Bernadette Pretzl, Jule Paul, Diana M. Krigar, Lorenz Uhlmann, Peter Eickholz & Bettina Dannewitz Comparison of Two Different Sampling Methods for Subgingival Plaque: Subgingival Paper Points or Mouthrinse Sample?

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Prevotella histicola.

1. Oral Microbiol Immunol. 1987 Sep;2(3):142-4.
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To evaluate the influence of sampling on the microflora, plaque samples were also taken twice at 2 teeth without therapy with PD of 3 to 5 mm (negative control). Collection of subgingival plaque samples with paper points is the common method for determination of periodontal pathogens. 21,24, 25, 27,28 However, sampling subgingival plaque with paper points After AT clinical examinations were performed and subgingival plaque was sampled from the same four sites as prior to AT (ASPRE) as well as from the four deepest sites after AT (DEEP).

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tively characterize the total culturable subgingival-plaque flora of one dog with ligatures and one dog without. Thus, at numer- ous time points, plaque samples Every individual was examined clinically and microbiologically using the CPITN index and collecting subgingival plaque samples. Each participant received a Periodontal parameters were recorded and deoxyribonucleic acid was extracted from the subgingival plaque and coronary atherosclerotic plaque samples of the The subgingival plaque samples will be analyzed to define the microbiotic profiles of the patients and the CGF determined to define their inflammatory The amount of stannous fluoride in GCF and subgingival plaque samples will be analyzed.. Registret för kliniska prövningar. ICH GCP. H2S production was measured with the bismuth method and subgingival plaque samples were analyzed for the presence of 20 bacterial species with the Subgingival plaque samples are also collected for microbiological analysis of the microbiota with focus on hydrogen sulfide producing bacteria. Samples are Subgingival plaque samples (SPS) and stimulated saliva samples (SSS) were periodontal outcome, periodontitis, saliva sample, subgingival plaque sample METHODS: Subgingival bacterial plaque was collected from women > or =6 The odds ratio of having P. aeruginosa at levels > or =1 x 10(5) in the sample and Fig.4. PCR analyses of subgingival plaque samples from subjects with and without bad breath.

Two weeks later irrigation procedures were 7 Feb 2017 The hypervariable region V4 of the 16S rDNA gene was targeted to explore richness and diversity of subgingival plaque samples [11]. Periodontitis is an attachment loss and inflammatory disease mainly caused by bacteria pathogens in subgingival plaque caused by immune-inflammatory periodontal disease; supragingival plaque; supra- and subgingival plaque samples from periodontitis subjects.